A Happy New Year and A New Beginning

A new year is upon us and with it comes new possibilities. This year we will discover even more miraculous properties of cannabis. From cancers to other ailments we already know the capabilities that cannabinoids possess. We have seen two states move forward beyond medical needs and legalize for recreational use. With that comes the hope that it will make it easier for patients to obtain the necessary strains they require.
We need to keep in mind that it is not simply using cannabis but using it correctly that will achieve desired results. As we have discussed before each strain possess different compounds or cannabinoids. We have already seen 3 strains genetically mapped, discovering a variety of previously unknown compounds and it will be exciting to see more added to the list.
We have seen so many advances in the delivery methods used for patients. We are no longer only left with smoking. For patients there are very real solutions.
We have seen countless stories of patients who were unable to eat, unable to control their shaking or seizures who have taken only a small amount of cannabis and have been able to control their symptoms. We have also seen in studies where cannabis has slowed or stopped cancer growth. It is no longer deniable that cannabis has real medical use.
I am hopeful that with the advancement of Washington and Colorado’s legislation as well as the other states that have set up medical provisions that we will continue to see the path cleared for all states and ultimately all patients to have access to not only cannabis but quality and variety of strains. Individualized care is key and will only come about for the masses if it becomes legally available. We have suffered for too long. We have lost too many loved ones unnecessarily. Now is the time to act! Call, e-mail or write your representatives and urge them to work towards the common goal of providing for all a safe natural alternative to big Pharma’s poison.

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Becky’s Story

It started out as coming home from Calif. as my kids were grown and I was sick. I had done Infer-on treatments twice, once with UNC Clinical trial then in Calif. I did 54 weeks started in 2010. It about killed my mind, body and soul. Luckily being Calif. it was no problem smoking or eating the Cannabis. I ended up on IV’s fluids for almost 4 yrs. most were in an infusion center at our local Hospital in Calif. I remember my nurse Abby well. Spent Christmas eve, New years Eve together. My Belly had been scarred bad. I couldn’t drink water for years. We drove me back in my rig in 09. My father had a set up for me to finally relax and heal me. Landed in the hospital 5 hours from home bad angina attack. Finally made it here on April 19th 2009. I even had to leave my 17 yr. Homecoming Queen, I missed so much. Cancer struck my Father Sam Haines Ret. Navy, Magna Cum Laude Honor Student Psychology, Master Gardner, Herbologist, and many other things a few months later. He always said the absetos from the Subs. would take him out. He didn’t want money for that, I respect it. He loved his USA. We lost him 3 months after I came home. A few months later I felt a sharp pain in my left lower belly it took me to the ER 24 hrs. later. Was the first time I had ever heard the word Cancer for me. It was a mass about the size of an Apple on my left Ovary. Six weeks later it had grown to 2 lbs. Took blood no Ovarian Cancer I was found, my OB did some major surgery to remove it and other organs. I woke up hearing it was bad, rare they had to send it to Duke the pathology. That is where my night mare begin as I was pretty much on my own here.
Came home, paid someone to bring me with 34 staples in my gut and a backed up septic tank, still have leach line issues. 3 weeks later I finally get a call. Musinous Adenocarcinoma 2 lb. Mass and Unknown Primary. Very Rare only 600 cases in the States a year. I found only 2 hospitals treated this one in San Diego and Wake Forest. Thought I was in, emailed Dr. Levine my Pathology, he quickly replied stated yes they do treat it, not mine, I was co-morbid. A term for med-care. If a person has 2 or more chronic conditions they won’t put the money in them. The Surgery was called MOAS a 23 hr. surgery cutting out 1/2 colon, 1/2 intestines and all lump nodes appendix and also HEPIC chemo. where they run warm chemo. in your guts sew you up and leave it for a few hrs. this giving me maybe 5 yrs to live. Duke jumped in, it was my CEA’s that were elevated the marker for Colon Cancer. Did a round, it about killed of Taxol. I lost my in home care I had since 2001 to the NC’s health plan. I did eat cannabis and smoke the whole time during the chemo. I could eat a chili dog had weird cravings while others were puking. Did have the water issue and my belly I had to look out for dehydration, I don’t think right when it happens those around me have signs. It didn’t get caught quite a few times, once I laid in my bed devacating on myself for 5 days, had Kloe looking out the window , I remember I thought it was snowing and no one could see us in here dying, wow. Sad looking back. My family tried , came in washed me up, bless their hearts. at one tine I even told Sam to get Kloe she had been eating chemo and was glowing green, he did it. I can give instructions. We got through it.
I had researched the Cannabis Oil as a cure, I believe in our science of Plants and it has always been a big part of my life, my father shared the history and health with me. Many other things but that is another story, If I remember right he was one of the Original ones in the first NORML he believed it should have been free, he fought for it. I’m no activist always;ys felt it was a gift to us, I choose to live off Grid I’ll get there again. I lost that in 99 due to Hurricane Floyd the flood. The oil I knew it would work, my children they only science they believed in was mans, I was forced into the chemo. it did no good on the numbers came back a tad more elevated. I did a search of the property as Dad told me he had material for bakeables wow was I surprised there was my oil meds. He had grew it he was going to compost, I told him to save it til I got back there ..lol..I guess it was part of my inheritence. Some one showed up from NCCPN she runs a differnt one now CCC I believe. Spent the first 3 months in a good Cannabis coma almost finally had a little help to feed me, I could rest, Rick Said that for it to work I must rest. I gave in. I have a list of 20 Chronic Conditions I have been pinned with. I knew the endocanabonoid system but had no idea I could feel this dang good.
Within 6 weeks I could tell a difference in my eating and resting I was able to shower again on a regular basis eating well. I took a lot of oil! Tumors started leaving I had nodules unknown stuff in my lungs they cleared. The drs. were amazed as we sat there and giggled they were lost to this science, I told them the whole time what I was doing, we preached Cannabis at Duke, eating my little green cookies everywhere . Around 6 months ago I had been studying food a long time, believe in our organics was almost there once, then got sick. I started this little crazy diet a friend mentioned also tips from my friend Tamara she cleared her Cancer through foods just wrote a book the fist print sold out, Alternative Health Solutions. I’ll post a link with this. 12 colors a day, it was simple to me, I needed simplicity. Lost so much, reading, writing, seeing, driving, etc. The GMOs are killing us also, I hope to climate all one day, also I’ll wear only hemp once I can shop for my clothes again, using 7th generation Baby products for the skin and clothes washing, dish washing all natural stuff. There were so many scripts I had been on since the Interferon treatment. I was ready for the pills to be gone too. I became quiet good at the oil , good shit, I jacked up my dose to detox off this stuff processed foods and the rest of the meds. I was on. I didn’t think I had to tell anyone what I was doing, it was my world here. The family reacted quite different to the natural healing. They had never heard me talk about Jesus before, I dig him. What happened to my health is short of a miracle all conditions cleared, Cancer, Fibro. Chronic Pain, Fatique, high blood pressure, high sugar, Epstien Barre, angina, spastic esophagus, chronic thrush had took my taste, also candida bad on my skin, Ptsd, Anxiety, Hyper-activity I’ll get that list there are more, even have chronic marijuana abuse, I’ll keep that one. There are 2 pills I have to take, I tested it, for hyperprotienlypidemia 4, a genetic blood disorder. The only meds .I will take, or my trich
Around 2 months ago I stepped out in our World, got my hair done, new clothes, the bloat from treatments just fell off with the oil like magic! I had no idea what would come of me coming out of this Corporate Greed. I ended up with an infection, it started a change for me with our local Hospital who I had standing orders for fluids at. I usually carry a book so they can see my condition if they can’t understand me, I come around after a bag or 2. I said ” I’m not from around here, I need fluids my family has sucked me dry.” Next thing I know I was being stripped of clothes and rights, the infection or fluids not treated. They shot me up with dope and knocked me out. I awoke in what they call here the observatory, wow, worse then jail, been there one night..lol. they see if you need to be in psych. took me 2 days to get my I rights back. I cleared the infection with the veggies and water brought to me in there I made them wait on my ass hand and foot, since they had me. I had to take a drug called Septra for Schizophrenia and shut up for 3 hrs before they let me out the door I conformed. I knew an injustice had been done I was going to let it go, but they got me again.
On Dec. 2nd, my daughter, she got worried, so was I. I had did so much of my oil i need to get some more done, no one understood what I was up against, I didn’t feel I had to explain myself as long as I was getting better. The Fytanal patches, celexa they gave me for crying during chemo. , the lortabs, Valiums I was ready to get them out of my system for good. I don’t need a pain pill today for the fibromyalgia. i did show my ass, I can’t rest with knots in my gut, things I need to get out with the family and community, no one fully understand how close I’ve been to death quite many times done with that shit. Autumn hadn’t seen me since we got me thru the chemo. Placed a 911 call to my ass to check on my welfare. I was already set and at the beach with my stuff getting ready to rent a place and relax a month, yes they found me there too 4 cops and ambulance pulled in the yard. The law can do that if they feel you have a mental heath, Holistic healing is not heard here, hardly. I told that Dr. Hussini, I had stepped out of the medical society I only suffer from PTSD as far as my head is concerned. He told me I could no step out of their system. watch me.
I’m really grasping what happened next. I’ll go into more detail later but it still hurts as I saw so much brutality. I was driven and handed over to the the psych. ward. 6 cops and 2 attented to tie me down in a seclusion room and shoot me up with drugs. This went on till the 20 of Dec. I fought that stuff hard. I would no conform. I wore my Army Creed shirt, taught everyone to say Pledge allienge I had to draw it out. Talked about the Cannabis the medical bill, they lock folks in our county up if they have a narcotic benzo or cannabis in your system and dry you out. When I left there the recovery counselor who was tough..lo. I heard tell our people yes smoke a joint, its ok, That felt good, things did change up there. I did finally conform as I wasn’t going to get out, took their drugs and the lituim this time they said I was bi-polar. no labels here, a little eccentric they thought I had grandiose ideas, which I don’t think anything is impossible. We are going to free our weed in the South. I feel it. I had to eat their foods, made me sick, sleep so many hours, I’m on my own schedule is my right. I have been court ordered to take the meds. After my release 2 days later I went back. I wanted them not to keep me on those drugs, I was able to release with my signature that Observatory on the 23rd of this month with my own signature yes. I won that mess. I do feel that the Hospital will be in my lawsuit pending the county. Here is what happened while I was away from my home.
Since I wasn’t home they bursted right in my door. I don’t worry about my cannabis here, or my guns or anything for that matter I did nothing wrong. I had my Christmas weed out, maybe 10 grams, I had left buckets soaking in the bathroom, the didn’t mess with that, they were kind of freaked on my big vial of left over oil. It was empty. Many drugs in the house, a grow room my dad grew veggies in, many guns, I had my tax money put up , they took all my stuff while they figured out I was no harm to a soul not a grower or dealer of any kind. Robeson here wants this Cannabis free, its taking up their courts and recovery for the real addicts and Alcoholics. We had yet to clean out my house from all the scripts. As I sit now I’m ok with what it took to get me here. I gotta go to court to get my stuff back my guns and money. My cable which was my connection to the world all cut turned off someone didn’t pay my bills, I came home to my car egged with my own eggs, inside and out that one bit I know who did it. My dang Kloe, I lost her, 2 of my hens, I’ve been having to be on-line outside I have enjoyed the freedom.
I’d like to get the ball started rolling on education here , in my side of the State 75% are stil without internet. We have to get something to them. I was going to front this part my self financially I had 2 land sales going on. I lost them both with my phone. HB 577 needs to be in every ones face if its going to pass in a year. It should have already been 3 years ago. I have been begging for help. We are going to run a fund for now. I’m putting in about my last 100 bucks to get it rolling. That way I can get the printed material they have been begging for in their hands at least in my county Columbus and Brunswick any others can contact me. I hope to have a link for those pamphlets soon. I can’t create them on my puter don’t have the program but have help for that once I get or info. more narrow down, Also putting in about those foods and gmos just a little ed. its not available here. Very sweet runs thru veins here, dying of Cancer all over the place Genocide is what I call it. I’m leaving on that one. Have a Bless day. Peace be with you all. yes, my medicine, my Cannabis OIL! I had enough to last me for a year, til it was free to grown my own weed! My friend Donnie destroyed all for my protection. Dang, I feel I may could have beat the case and won that shit back. Out of my hands I’m ok. The foods and I smoke and eat my cookies still. Spaz is a nervous little thing she suffers from PTSD also. Do be careful with that watched a video last night out of Co. they are rushing knocked out stoned dogs to the Vets.
Magic Carpet Ride
http://www.youtube.com

Cannabis Less Harmful Than Current Drug Therapies?

Many when faced with a serious illness fight for their life but ironically are prescribed medications that in fact not only cause harm but also are capable of taking their life.  Many prescription side effects are quite lengthy.  Those that are on prescriptions long-term end up requiring even more medication down the line to combat the side effects from the first drug.  With all that in mind patients are looking for a safer alternative solution.

From cancers to anxiety many prescribed therapies are not only dangerous but can cause death.  Chemotherapy has come a long way from the early days but it is still a drug that ravages the body.  By contrast research has shown that cannabinoid therapies induce programmed cell death in cancerous cells.  Cannabinoids as is widely known are praised for their ability to alleviate without any reported deaths throughout its historic, several thousand year use.

Although the official stance from the government is that cannabis has no accepted medical value science tells us otherwise.  Below you will see a study that weighs the harm of accepted drug therapies such as opioids.  As always I encourage all to do their own research and please feel free to share ~ Cherry Girl

Prescribing Cannabis For Harm Reduction 2012
Neuropathic pain affects between 5% and 10% of the US population and can be refractory to treatment. Opioids may be recommended as a second-line pharmacotherapy but have risks including overdose and death. Cannabis has been shown to be effective for treating nerve pain without the risk of fatal poisoning. The author suggests that physicians who treat neuropathic pain with opioids should evaluate their patients for a trial of cannabis and prescribe it when appropriate prior to using opioids. This harm reduction strategy may reduce the morbidity and mortality rates associated with prescription pain medications.

U.S. Government Destroying History: Why We Must Save Cannabis Plant Genetics From Extinction

Strains such as Kush have been made notorious through songs and celebrities professing their love for the strain.  Those that have experienced Kush know why it is so famous.  From Tahoe OG Kush to Afghan Kush the high is memorable.  Cannabis is not a new plant created in a lab but rather has been used for centuries as medicine, religious herb and for recreation.  Research proves more every day how vital plant genetics are in finding the right compounds to combat illnesses and find cures.  Genetics is something that isn’t often thought of when discussing the War on Drugs but it has definitely been affected by it.

Some cannabis genetics are thought to have come from Providences in China and spread throughout the Silk Road while others originate in the Middle East.  There of course was the large movement through the 60s and 70s that helped spread and create new cannabis strains through people exchanging seeds and ganja.

With new genetics infiltrating the gene pool in the States new plant strains emerged.  Hybrids also came out of the mix.  People discovered that by crossing different strains they could breed out the undesirable traits while promoting higher potency and better growth stamina.  By cross breeding not only Indica with Indica but breeding for balance we have seen hybrid strains that have the pain relief of an Indica but with the “upper” functioning high of a Sativa.  Genetics play a role in growth, mold/pest resistance and of course potency.  

Blueberry strains are known for their social anxiety and pain relief as well as helping with digestive disorders and have spawned notorious strains like Blue Cheese, Blue Dream, Old-Time Moonshine and Blue Haze .  DJ Short created the Blueberry strain by crossing his Afghani Indica, brought by some growers from California and his Purple Thai.  The female pedigree of his Purple Thai is his highly potent all-around champion, “Juicy Fruit” Highland Thai female. It packed a very sweet tropical punch and an intense high that lasted for many hours. The second plant was a Purple Thai out of Oregon, Ohio. This plant was a cross between an outstanding Highland Oaxaca Gold and a very freaky Chocolate Thai. The hybrid was very dark colored and would express a deep royal purple color at the slightest exposure to cold. The finished product was equally tasty and strong as the Juicy Fruit female.

The War on Drugs forced cannabis use underground.  Cannabis began being bred for bag appeal and what would bring the best sales.  Growers began looking for traits that would bring the biggest yield with the shortest flowering time.  Medicinal qualities were disregarded.    For decades it was thought that the compound THC was responsible for all of the effects cannabis provided.  We now know of CBD which is actually responsible for pain relief and has been found to help with insomnia, anxiety and Alzheimer’s.  CBD was another casualty of the war with most of today’s strains registering less than 0.5%.  With the War on Drugs ongoing for over 40 years countless plants have been destroyed with the government’s actions forever destroying precious genetics.

Although cannabis has been around for thousands of years it wasn’t until the summer of 2011 that the genetics of the cannabis Sativa and Indica were mapped.  We know that there are hundreds of strains that have come from the original heirloom landraces.  Today many patients and citizens alike have the option of what type of relief or high they are looking for.  From the euphoric highs of the Sativa to the amazing pain relief that Indicas bring the options are endless.

Everyday we have new research that is released that shows the anti-cancer properties of cannabinoids.  Now more than ever we understand why we need to preserve the genetics of all cannabis plants.  We know that we can use different strains to treat a wide range of illnesses and afflictions.  We also know that cannabis is not a one-size-fits-all type of plant.  You need to have the correct strain containing the right balance of compounds to reach the desired effects.

We have more proof than we have ever had before showing the medical benefits even on a molecular level.  We are beyond smoke when taking about cannabis therapies.  There are a wide range of options when talking about uses.  Tinctures, edibles, concentrates, oils, topical lotion, capsules, vaporizers and more.

Support is at an historic high with medical use over 75% and legalization for adults over 21 at 50%.  States that have enacted decriminalization have found favorable outcomes.  States that have enacted medical statues have found large increases in tax income as well as economic growth in the communities.

If cannabis is allowed to thrive imagine the possibilities.  Imagine 5, 10, 15 years from now how much we will know about genetics, compounds and treatments for cancers.  Imagine all the possibilities if we could openly and safely exchange genetics and end products.  How many people could find relief?  How many could save their life?

It is time that we stand up and protect cannabis genetics.  We need to stop the violent raids by government officials.  The government needs to change their stance on cannabis therapies.  Now more than ever it is time we stop the continual attacks on patients, crops and our heritage.  The War on Drugs needs to end.  It has been a failed war for over 40 years that has forever changed the history of cannabis.  Those that have kept cannabis genetics alive even in the face of persecution and imprisonment thus passing down history should be celebrated.  Urge your representative today to show support for ending the failed approach to dealing with cannabis.

Cannabis, Cancer And Children:Why We Must Give Parents A Choice

There is something special about children that brings a smile.  Some parents wait a long time to be able to have that special time to cradle their child, gazing into their eyes.  Imagine being the parents faced with the reality that your child has cancer.  For over 10,000 parents each year that is the case.  Vigilantly staying by their child’s side through countless shots, painful procedures, body wrenching medications and worst of all watching as their child withers away before their eyes.  The spark slowly going out leaving behind a weak body.  Most would try anything to save their baby.  

Current therapies  such as chemotherapy are not precise measures.  Rates of success in comparisson with side effects make some wonder why alternatives are being stifled.    In contrast there is mounting evidence of the anti-tumor effects of cannabis compounds.  Cannabis has been praised for its large window of error since cannabis does not cause death.

Most in the cannabis community know who Cashy Hyde is.  He is the little boy who’s father treated him with cannabis oil and who today is cancer free.  Cashy has undergone extensive treatments that ravaged his tiny body.  His father watched as the light faded and decided to save his son.  He fed Cashy Rick Simpson Method Cannabis Oil through his feeding tube and miraculously watched his son bounce back to life.  Within days they noticed a difference.

Cashy is not alone.  There are millions of others that have been helped and cured with cannabis.  The discussion of cannabis is beyond smoke.  Cannabis research has shown that by targeting the Endocannabinoid system with the right combinations of compounds you will receive the desired effects including inducing cell death in cancer.

With so much more scientific medical research as proof the majority of the country is asking why we still pursue prohibition with a vengeance.  California has been helping patients since 1996.  Last year we saw a flood of stories where federal agencies terrorized local communities with their SWAT style raids.  In doing so they cut off supply of much needed medicine for patients just like Cashy.

We must do something to give parents an option to save their children.  When parents are told there is nothing more that can be done should we really take away hope.  Should we incarcerate parents for possessing a plant, one that science proves is lifesaving?  We need to bring sanity back into the equation.  We must change the laws.  Me must challenge the “way its always been”.  It is time to allow parents the right to decide for their children.  Several states have proposed or pending legislation.  Contact your state representatives and urge them to help change the future for our children.

Newly Released Studies Show Protection and Regeneration of Brain Cells with Cannabis Therapies

There are over 1.7 million cases of severe brain injury that are reported each year.  These injuries can be life altering for many but thanks to the hard work from the science and medical communities we are learning more about new treatment options.  Cannabis has been discovered to help with cell regeneration and repair in the brain.  Below you will see newly published studies where  cannabis has been applied to help with brain injuries. Truly exciting!!   As always I encourage all to do their own research as well and please feel free to share ~ Cherry Girl

Early Survival of Comatose Patients After Severe Traumatic Brain Injury With the Dual Cannabinoid CB1/CB2 Receptor Agonist KN38-7271
Despite many drug trials, no substance has yet been identified that improves the outcome of severe head injury. The dual cannabinoid CB1/CB2 receptor agonist KN38-7271 mediates potent neuroprotection in animal models. We describe here the first randomized, double-blind, prospective, placebo-controlled clinical phase IIa proof-of-concept trial to investigate the safety, pharmacokinetics, and potential efficacy of a cannabinoid receptor agonist in humans.  Conclusions KN38-7271 appeared beneficial in the acute early phase of the comatose patient after a head injury. Its use was safe and well tolerated by patients.

Cannabinoids As Neuroprotective Agents In Traumatic Brain Injury
Cannabinoids of all classes have the ability to protect neurons from a variety of insults that are believed to underlie delayed neuronal death after traumatic brain injury (TBI), including excitotoxicity, calcium influx, free radical formation and neuroinflammation.

Cannabidiol Administration After Hypoxia-Ischemia To Newborn Rats Reduces Long-Term Brain Injury and Restores Neurobehavioral Function 2012
Cannabidiol (CBD) demonstrated short-term neuroprotective effects in the immature brain following hypoxia-ischemia (HI).   In conclusion, CBD administration after HI injury to newborn rats led to long-lasting neuroprotection, with the overall effect of promoting greater functional rather than histological recovery.

Cannabidiol Reduces Brain Damage and Improves Functional Recovery After Acute Hypoxia-Ischemia In Newborn Pigs 2011
Newborn piglets exposed to acute hypoxia-ischemia (HI) received i.v. cannabidiol (HI + CBD) or vehicle (HI + VEH). In HI + VEH, 72 h post-HI brain activity as assessed by amplitude-integrated EEG (aEEG) had only recovered to 42 ± 9% of baseline, near-infrared spectroscopy (NIRS) parameters remained lower than normal, and neurobehavioral performance was abnormal (27.8 ± 2.3 points, normal 36).   In conclusion, post-HI administration of CBD protects neurons and astrocytes, leading to histological, functional, biochemical, and neurobehavioral improvements.
 

Cannabis Has Cancer In The Cross-Hairs. Utilizing The Endocannabinoid System To Kill Cancer

It seems that these days the word cancer is everywhere.  From billboards encouraging donations to charities to news headlines to loved one effected by cancer most people are familiar.  There are more than 200 different types of cancer that can develop in over 60 different organs in the body.  Current therapies include poisoning the body in hopes that it will also kill the cancer.  Cannabis is used to help counteract the side effects of these therapies.  However new research points to cannabis as the compounds that can actually cause cancer cell death, essentially curing cancer.  There are countless studies that have been published showing the benefits of using the endocannabinoid system to target cancer cells with cannabinoids.  From that research we have found where using the endocannabinoid system has proven vital in fighting cancer.  Below you will see several studies that show how using the endocannabinoid system can be used in targeting cancer.  Please feel free to share and help others see that using cannabis is beyond the smoke.  I encourage all to do their own research as well ~ Cherry Girl

The Endocannabinoid System in Cancer-Potential Therapeutic Target?
Endogenous arachidonic acid metabolites with properties similar to compounds of Cannabis sativa Linnaeus, the so-called endocannabinoids, have effects on various types of cancer.    Remarkably, these effects may be selective for the cancer cells, while normal cells and tissues are spared. Such apparent tumor cell selectivity makes the endocannabinoid system an attractive potential target for cancer therapy.

Targeting the Endocannabinoid System For the Treatment of Cancer–A Practical View
In recent years, considerable interest has been generated by findings that cannabinoids not only have useful palliative effects, but also can affect the viability and invasivity of a variety of different cancer cells.   It is concluded that cannabinoids (or agents modulating the endogenous cannabinoid system) are an attractive target for drug development in the cancer area….

The Endocannabinoid System As A Target For the Development of New Drugs For Cancer Therapy
Studies on the main bioactive components of Cannabis sativa, the cannabinoids, and particularly (THC), led to the discovery of a new endogenous signalling system that controls several physiological and pathological conditions: the endocannabinoid system. Recently, evidence has accumulated indicating that stimulation of cannabinoid receptors by either THC or the endocannabinoids influence the intracellular events controlling the proliferation and apoptosis(cell death) of numerous types of cancer cells, thereby leading to anti-tumour effects both in vitro and in vivo. This evidence is reviewed here and suggests that future anti-cancer therapy might be developed from our knowledge of how the endocannabinoid system controls the growth and metastasis of malignant cells.

Endocannabinoid System Modulation In Cancer Biology and Therapy
Endocannabinoid system modulation in cancer biology and therapy. The discovery of the endocannabinoid system… led to the development of therapeutic agents related to either the stimulation or antagonism of CB1 and CB2 cannabinoid receptors… evidence suggests that agonists of cannabinoid receptors, which share the useful property to discern between tumor cells and their non-transformed counterparts, could represent novel tumor-selective tools to treat cancer in addition to their already exploited use as palliative drugs.

Use of Cannabinoid Receptor Agonists In Cancer Therapy As Palliative and Curative Agents
“Use of cannabinoid receptor agonists in cancer therapy as palliative and curative agents… Cannabinoids (the active components of Cannabis sativa)… development, exploration and use of cannabinoid agonists for cancer therapy, not only as palliative but also as curative drugs.

Changes in the Endocannabinoid System May Give Insight into new and Effective Treatments for Cancer
Marijuana and its derivatives have been used in medicine for centuries… cannabinoids might be effective anti-tumoral agents because of their ability to inhibit the growth of various types of cancer… Evidence suggests that the endocannabinoid system may be dysregulated in a number of cancers… the endocannabinoid system exerts a myriad of effects on tumor cell growth, progression, angiogenesis, and migration. With a notable few exceptions, targeting the endocannabinoid system with agents that activate cannabinoid receptors or increase the endogenous levels of AEA may prove to have therapeutic benefit in the treatment of various cancers.

Beyond The Smoke: The Anticancer Properties Of Cannabis

Cannabis has many amazing healing properties that utilize the endocannabinoid system to reach target areas.  Scientists use this knowledge to target certain aspects of a condition depending on the symptoms.  It is well known that cannabis use can help cancer patients during the course of their battle, from increased appetite to pain relief.  Scientists have been uncovering cannabis’ anticancer effects as well.  This promising research is clearing the way so that one day we will fully understand this miracle plant.  Below are studies where science has looked at cannabis therapy when applied to cancer cells.  Please feel free to share and as always I encourage all to do their own research.  Please note that a lot of these are NEW 2012 released research which is really exciting! ~ Cherry Girl

CANNABIDIOL AS POTENTIAL ANTICANCER DRUG
Over the past years, several lines of evidence support an antitumorigenic effect of cannabinoids including Δ(9) -tetrahydrocannabinol (Δ(9) -THC), synthetic agonists, endocannabinoids and endocannabinoid transport or degradation inhibitors.

Non-THC Cannabinoids Counteract Prostate Carcinoma Growth In Vitro and In Vivo: Pro-Apoptotic Effects and Underlying Mechanisms 2012
Cannabinoid receptor activation induces prostate carcinoma cell…In LNCaP cells, the pro-apoptotic effect of CBD was only partly due to TRPM8 antagonism and was accompanied by down-regulation of AR, p53 activation and elevation of reactive oxygen species. LNCaP cells differentiated to androgen-insensitive neuroendocrine-like cells were more sensitive to CBD-induced apoptosis. Conclusions. These data support the clinical testing of CBD against prostate carcinoma.

Role of Lipid Rafts/Caveolae in the Anticancer Effect of Endocannabinoids 2012
The endocannabinoid system comprises the cannabinoid receptors type 1 (CB1) and type 2 (CB2), their endogenous ligands (endocannabinoids) and the whole apparatus… drugs targeting the endocannabinoid system might be used to retard or block cancer growth… Perturbation of lipid rafts/caveolae may in fact represent a useful tool for the development of a novel therapy for endocannabinoids-related diseases, such as cancer.

Update On the Endocannabinoid System As An Anticancer Target
Recent studies have shown that the endocannabinoid system (ECS) could offer an attractive antitumor target.  One direction that should be pursued in antitumor therapy is to select compounds with reduced psychoactivity. This is known to be connected to the CB1 receptor; thus, targeting the CB2 receptor is a popular objective. CB1 receptors could be maintained as a target to design new compounds, and mixed CB1-CB2 ligands could be effective if they are able to not cross the BBB. Furthermore, targeting the ECS with agents that activate cannabinoid receptors or inhibitors of endogenous degrading systems such as fatty acid amide hydrolase inhibitors may have relevant therapeutic impact on tumor growth. Additional studies into the downstream consequences of endocannabinoid treatment are required and may illuminate other potential therapeutic targets.

Use of Cannabinoid Receptor Agonists In Cancer Therapy As Palliative and Curative Agents
Emerging evidence suggests that agonists of cannabinoid receptors expressed by tumour cells may offer a novel strategy to treat cancer. In this chapter we review the more recent results generating interest in the field of cannabinoids and cancer, and provide novel suggestions for the development, exploration and use of cannabinoid agonists for cancer therapy, not only as palliative but also as curative drugs.

Inhibitory Effects of Cannabinoid CB1 Receptor Stimulation on Tumor Growth and Metastatic Spreading: Actions on Signals Involved in Angiogenesis and Metastasis
Stimulation of cannabinoid CB1 receptors by 2-methyl-arachidonyl-2′-fluoro-ethylamide (Met-F-AEA) inhibits the growth of a rat thyroid cancer cell-derived tumor in athymic mice by inhibiting the activity of the oncogene product p21ras.  Three weeks from the paw injection of 3LL cells, Met-F-AEA reduced significantly the number of metastatic nodes, in a way antagonized by SR141716A. Our findings indicate that CB1 receptor agonists might be used therapeutically to retard tumor growth in vivo by inhibiting at once tumor growth, angiogenesis, and metastasis.

Good News For CB1 Receptors: Endogenous Agonists Are in the Right Place
Good news for CB1 receptors: endogenous agonists are in the right place” and “CB1 receptor agonists might be used therapeutically to retard tumor growth in vivo by inhibiting at once tumor growth, angiogenesis, and metastasis.

Breaking News: Cashy Hyde Free From Cancer

The Cashy Hyde case has been one that has been closely followed by many in the cannabis community that believe in the healing power of cannabis.  Below is the breaking news released yesterday.

On Wednesday, February 22, 2012 Cash Michael Hyde was in Salt Lake City for a follow up MRI to check the status of his brain tumor. A PNET brain tumor he has been battling for almost 2 years.

For the second time in a row, Cashy has beat his tumor, and is in remission.

The Boy of Steel is Cancer Free!

How many people do you know that beat cancer once, nonetheless twice?

At THREE years of age?

Congratulations and hugest of blessing upon his parents Mike and Kalli for being the strong parents they are, and fighting so diligently to save their son’s life with an alternative option.

Thank heavens for the cannabis oil that saves Cashy’s life.

Be vigilant in your duties Pioneers……your efforts are important to people just like Cashy and his family EVERY day! Act responsibly! Keep up the great work!

And to all the politicians out there…….please support the Hyde family, and many other families out there just like them.

We are here to save children’s lives, not take them away.

Love and blessings to all…..I’m off to celebrate on the ride back to Montana with the Hyde’s.

I love them all so very much. Better be a BIG box of Kleenex on board as we will all be so HAPPY!

For more information please view original source or visit The Cash Hyde Foundation.

Monumental Achievement – Cannabis Plant Genetics Mapped

In 2011 we saw cannabis all over the news.  We saw raids, booming industries, more states push for and more doctors taking a stand for legalization.  Cannabis has been in use by humans since at least 2737 BCE with Chinese Emperor Shen-Nung recorded prescribing it for a number of ailments.

There are many mysteries and controversies surrounding the cannabis plant.  Science has been trying to unravel them for years.  In 2011 we had a monumental breakthrough that quite literally could change the course of history.

Kevin McKernan has stacked up a long list of big accomplishments at just 38.  He is the owner of Medicinal Genomics with a lab in Amsterdam for researchers to freely study the cannabis plant.  Kevin was part of the Human Genome Project for four years.  He also worked on a robotic system that could handle He also developed methods to process DNA 100,000 times faster.

With a man who’s resume is that impressive you have to ask yourself why would a guy like that get into the business of studying cannabis.  He did work on mapping tumors and after working with doctors and patients started to see that many cancer patients were using cannabis.  After looking into cannabis he saw that there was much more to this plant and that it did have medicinal value.

While working with oncologist he found that if you sequence a tumors DNA as well as the patient you will discover the differences which can then become a target for drug therapies.  With cannabis being mapped the same theory could apply, instead of using Big Pharma drugs you could use Cannabinoids.

With both the Indica and Cannabis plant genetics mapped science can now start to get a better grasp on how cannabinoids interact with our bodies.  When we can successfully target each person illness with specific cannabinoids we will effectively understand the science behind the curing powers of cannabis.

Science will finally be able to confirm what millions have known for thousands of years.  Today we know more about THC, the psychoactive ingredient and the CBD, non-psychoactive ingredient and how the synthases of each are the genes that code for an enzyme that helps fold the hydrocarbons into Cannabinoids.

What controls the other 83 cannabinoids and other hundreds of terpenes is still unknown.   Many genes that were bred out in search of higher THC content since the government prohibited cannabis use, will need to be brought back through selective breading.

This is one more step in our fight for legalization.  With scientific proof the government will have no choice but to recognize cannabis has medicinal value.

Even the national Cancer Institute briefly posted a page that listed cannabis as a pain reliever and anti-nausea as well as possible anti-tumor effects.  The government made them take it down since this has only been proven in cell cultures and rats although it is currently in human clinical trials.

When dealing with chemotherapeutic drugs there is a fine line between killing the tumor and killing the patient.  Whats exciting about cannabis is that it is not fatal and very few drug therapies have such a forgiving window.

With all this new data in the coming years we will see our knowledge grow and hopefully result in legalization.  It will take all of us making our voices heard, that we should have a natural choice when treating our illnesses.  Kevin and his team have posted their findings online for all to look at.  Please feel free to share ~ Cherry Girl

Targeting Our Endocannabinoid System:Search For Nature’s Cure

The endocannabinoid system was discovered in the 1960’s and since then scientists have been hard at work trying to unravel the mysteries.  Below you will see studies showing how the endocannabinoid system within the human body is at the center for learning how to treat many illnesses and diseases. Please feel free to share the truth about the CURE ~ Cherry Girl

Endocannabinoids In The Immune System and Cancer
Although its action as an immunomodulatory molecule requires further characterization, modulation of the endocannabinoid system interferes with cancer cell proliferation either by inhibiting mitogenic autocrine/paracrine loops or by directly inducing apoptosis.   In conclusion, further investigations are needed to elucidate the function of endocannabinoids as immunosuppressant and antiproliferative/cytotoxic agents. The experimental evidence reviewed in this article argues in favor of the therapeutic potential of these compounds in immune disorders and cancer.

Endocannabinoid System Modulation In Cancer Biology and Therapy
The discovery of the endocannabinoid system and the recognition of its impact in a plethora of pathological conditions, led to the development of therapeutic agents related to either the stimulation or antagonism of CB1 and CB2 cannabinoid receptors…Endocannabinoid-related agents have been reported to affect multiple signaling pathways and biological processes involved in the development of cancer, displaying an interesting anti-proliferative, pro-apoptotic, anti-angiogenic and anti-metastatic activity both in vitro and in vivo in several models of cancer. Emerging evidence suggests that agonists of cannabinoid receptors, which share the useful property to discern between tumor cells and their non-transformed counterparts, represent novel tumor-selective tools to treat cancer in addition to their already exploited use as palliative drugs to treat chemotherapy-induced nausea, pain and anorexia/weight loss in cancer patients.

Possible Endocannabinoid Control Of Colorectal Cancer Growth
CONCLUSIONS: Endocannabinoid levels are enhanced in transformed colon mucosa cells possibly to counteract proliferation via CBRs. Inhibitors of endocannabinoid inactivation may prove useful anticancer agents

Cannabinoids, Endocannabinoids, and Cancer
Modulation of the endocannabinoid system by pharmacological agents in various cancer types reveals that it can mediate antiproliferative and apoptotic effects by both cannabinoid receptor-dependent and -independent pathways…the endocannabinoid system is a promising target for the development of novel chemotherapeutics to treat cancer.

The Endocannabinoid System As A Target For The Development Of New Drugs For Cancer Therapy
stimulation of cannabinoid receptors by either THC or the endocannabinoids influence the intracellular events controlling the proliferation and apoptosis of numerous types of cancer cells, thereby leading to anti-tumour effects both in vitro and in vivo

Dear Mr. President or Anyone Else Listening

I write to put a face to this war on medical cannabis.  There are things in life that I hope most never experience.  Having to hear the words that a loved one has cancer. Watching them slowly die from toxic chemo treatments. To see their eyes fade, the life shrinking away preparing for death.  But in all the misery there is hope from strangers. Strangers that risk their life to provide a life saving oil, one that you have deemed too harmful for the public. Despite this the oil is used and the fire in their eyes returns little by little and they come back from the brink.  The doctors say its a miracle but we know its a natural cure.  It is hard to believe that this life saving oil has no medical value when thousands have seen it with their own eyes.  Its hard to believe that this is so dangerous that it must be kept away from the public with hard consequences if obtained.  Its hard to believe that this natural cure is equated with heroin and other harmful drugs.  There are millions of silent users, including yourself, that have used it and not only survived but benefited from it.  It is not a new designer drug but has been used medicinally for thousands of years. Modern science has not unlocked all the mysteries of cannabis.  What we do know is promising and does point towards a cure for cancer as well as other illnesses.  We can not wait for modern science to figure out what millions already know.  We used to put people to death for saying the world is round, which in modern times would sound ridiculous but with cannabis prohibition restricting access to this natural cure, you are essentially condemning thousands of patients to suffer in pain or die from their illness.  We are all born of free will.  We are all born with the right to decide what is best for our own body.  Government has no business making medical and personal decisions for me or anyone else.   We should not be forced to watch our loved ones slowly and agonizingly die from government approved cancer treatments.  We should be able to pursue a natural treatment instead of only those the government pushes.  We can not stand by and watch as politics dictates how we treat our illnesses when patients lives are at stake.  We are all adults and we need to be treated that way.  We need responsible regulations.  We need to come together to ensure safe access for all. There is an opportunity here that is yours for the taking.  You can make a difference, you can right wrongs and stop the oppression of millions.  You have experienced cannabis surely you can see it is not the same as heroin or other schedule 1 drugs.  Millions of lives have been changed forever for simple possession.  Countless families turned upside down over a plant.  Its time for a change, its time for legalization!

Take Action Now!

Cash “Cashy” Hyde: Children’s Cancer and Cannabis

Hearing your child has cancer is one of the worst things a parent can be faced with.  Watching your child go through chemo can be a horrifying experience in itself.  When news broke that a father was treating his son’s cancer with cannabis people were shocked.  There was reactions all over the board but we watched as the oil worked.  Cashy, as he is known has been diagnosed with Malignant and Aggressive Cancer classified as a PNET Brain Tumor.  He was able to replace the toxic drugs he was on with cannabis oil instead.  Below are studies that show how cannabis treats Brain Tumors.  With more research we can prove that cannabis does have medicinal value and can greatly aid or cure many ailments.  These are published medical studies that I encourage you to research and share your findings with everyone.  Spread the truth!
Information gathered by David Worrell edited by Cherry Girl
Please visit the Cashy Hyde Foundation Website for more on this amazing little boy!   

Hypothesis: Cannabinoid Therapy For the Treatment of Gliomas?
‎”Cannabinoids induce apoptosis of glioma cells in culture… In addition, cannabinoid treatment inhibits angiogenesis of gliomas in vivo. Remarkably, cannabinoids kill glioma cells selectively and can protect non-transformed glial cells from death. These and other findings reviewed here might set the basis for a potential use of cannabinoids in the management of gliomas.”
“Hypothesis: cannabinoid therapy for the treatment of gliomas?”
Velasco G, Galve-Roperh I, Sánchez C, Blázquez C, Guzmán M.
SourceDepartment of Biochemistry and Molecular Biology I, School of Biology, Complutense University, Avenida Complutense, sn, 28040 Madrid, Spain.

Abstract
Gliomas, in particular glioblastoma multiforme or grade IV astrocytoma, are the most frequent class of malignant primary brain tumours and one of the most aggressive forms of cancer. Current therapeutic strategies for the treatment of glioblastoma multiforme are usually ineffective or just palliative. During the last few years, several studies have shown that cannabinoids-the active components of the plant Cannabis sativa and their derivatives–slow the growth of different types of tumours, including gliomas, in laboratory animals. Cannabinoids induce apoptosis of glioma cells in culture via sustained ceramide accumulation, extracellular signal-regulated kinase activation and Akt inhibition. In addition, cannabinoid treatment inhibits angiogenesis of gliomas in vivo. Remarkably, cannabinoids kill glioma cells selectively and can protect non-transformed glial cells from death. These and other findings reviewed here might set the basis for a potential use of cannabinoids in the management of gliomas.”
http://www.ncbi.nlm.nih.gov/pubmed/15275820

A Pilot Clinical Study of Delta9-tetrahydrocannabinol In Patients With Recurrent Glioblastoma Multiforme
‎”Delta(9)-Tetrahydrocannabinol (THC) and other cannabinoids inhibit tumour growth and angiogenesis.The primary end point of the study was to determine the safety of intracranial THC administration.Cannabinoid delivery was safe and could be achieved without overt psychoactive effects.The fair safety profile of THC, together with its antiproliferative action on tumour cells reported here and in other studies, may set the basis for future trials aimed at evaluating the potential antitumoral activity of cannabinoids.”
‎A pilot clinical study of Delta9-tetrahydrocannabinol in patients with recurrent glioblastoma multiforme. Guzmán M, Duarte MJ, Blázquez C, Ravina J, Rosa MC, Galve-Roperh I, Sánchez C, Velasco G, González-Feria L. Source Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, Madrid 28040, Spain. mgp@bbm1.ucm.es

Abstract
Delta(9)-Tetrahydrocannabinol (THC) and other cannabinoids inhibit tumour growth and angiogenesis in animal models, so their potential application as antitumoral drugs has been suggested. However, the antitumoral effect of cannabinoids has never been tested in humans. Here we report the first clinical study aimed at assessing cannabinoid antitumoral action, specifically a pilot phase I trial in which nine patients with recurrent glioblastoma multiforme were administered THC intratumoraly. The patients had previously failed standard therapy (surgery and radiotherapy) and had clear evidence of tumour progression. The primary end point of the study was to determine the safety of intracranial THC administration. We also evaluated THC action on the length of survival and various tumour-cell parameters. A dose escalation regimen for THC administration was assessed. Cannabinoid delivery was safe and could be achieved without overt psychoactive effects. Median survival of the cohort from the beginning of cannabinoid administration was 24 weeks (95% confidence interval: 15-33). Delta(9)-Tetrahydrocannabinol inhibited tumour-cell proliferation in vitro and decreased tumour-cell Ki67 immunostaining when administered to two patients. The fair safety profile of THC, together with its possible antiproliferative action on tumour cells reported here and in other studies, may set the basis for future trials aimed at evaluating the potential antitumoral activity of cannabinoids.”
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2360617/?tool=pubmed

Cannabinoids and Gliomas
“Cannabinoids, the active components of Cannabis sativa L., inhibit the growth of different types of tumor cells, including glioma cells. Cannabinoids seem to be selective antitumoral compounds, as they kill glioma cells, but not their non-transformed astroglial counterparts. On the basis of these preclinical findings, a pilot clinical study of Delta(9)-tetrahydrocannabinol (THC) in patients with recurrent glioblastoma multiforme has been recently run. The good safety profile of THC, together with its growth-inhibiting action on tumor cells, justifies the setting up of future trials aimed at evaluating the antitumoral activity of cannabinoids.”
Cannabinoids and gliomas. Velasco G, Carracedo A, Blázquez C, Lorente M, Aguado T, Haro A, Sánchez C, Galve-Roperh I, Guzmán M.
Source Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, 28040 Madrid, Spain.

Abstract
Cannabinoids, the active components of Cannabis sativa L., act in the body by mimicking endogenous substances–the endocannabinoids–that activate specific cell surface receptors. Cannabinoids exert various palliative effects in cancer patients. In addition, cannabinoids inhibit the growth of different types of tumor cells, including glioma cells, in laboratory animals. They do so by modulating key cell signaling pathways, mostly the endoplasmic reticulum stress response, thereby inducing antitumoral actions such as the apoptotic death of tumor cells and the inhibition of tumor angiogenesis. Of interest, cannabinoids seem to be selective antitumoral compounds, as they kill glioma cells, but not their non-transformed astroglial counterparts. On the basis of these preclinical findings, a pilot clinical study of Delta(9)-tetrahydrocannabinol (THC) in patients with recurrent glioblastoma multiforme has been recently run. The good safety profile of THC, together with its possible growth-inhibiting action on tumor cells, justifies the setting up of future trials aimed at evaluating the potential antitumoral activity of cannabinoids.”
http://www.ncbi.nlm.nih.gov/pubmed/17952650

Cannabinoids As Potential New Therapy For the Treatment of Gliomas
“Gliomas constitute the most frequent and malignant primary brain tumors. Current standard therapeutic strategies (surgery, radiotherapy and chemotherapeutics) for their treatment are only palliative and survival diagnosis is normally 6-12 months. Cannabinoids appear to be selective antitumoral agents as they kill glioma cells without affecting the viability of nontransformed counterparts. A pilot clinical trial on patients with glioblastoma multiforme demonstrated their good safety profile together and remarkable antitumor effects, and may set the basis for further studies aimed at better evaluating the potential anticancer activity of cannabinoids.”
Cannabinoids as potential new therapy for the treatment of gliomas.
Parolaro D, Massi P. Source Department of Structural & Functional Biology, Pharmacology Section, Center of Neuroscience, University of Insubria, Via A da Giussano 10, Busto Arsizio (VA), Italy. daniela.parolaro@uninsubria.it

Abstract
Gliomas constitute the most frequent and malignant primary brain tumors. Current standard therapeutic strategies (surgery, radiotherapy and chemotherapeutics, e.g., temozolomide, carmustin or carboplatin) for their treatment are only palliative and survival diagnosis is normally 6-12 months. The development of new therapeutic strategies for the management of gliomas is therefore essential. Interestingly, cannabinoids have been shown to exert antiproliferative effects on a wide spectrum of cells in culture. Of interest, cannabinoids have displayed a great potency in reducing glioma tumor growth either in vitro or in animal experimental models, curbing the growth of xenografts generated by subcutaneous or intratecal injection of glioma cells in immune-deficient mice. Moreover, cannabinoids appear to be selective antitumoral agents as they kill glioma cells without affecting the viability of nontransformed counterparts. A pilot clinical trial on patients with glioblastoma multiforme demonstrated their good safety profile together and remarkable antitumor effects, and may set the basis for further studies aimed at better evaluating the potential anticancer activity of cannabinoids.”
http://www.ncbi.nlm.nih.gov/pubmed/18088200

The Stress-Regulated Protein P8 Mediates Cannabinoid-Induced Apoptosis of Tumor Cells
‎”One of the most exciting areas of current research in the cannabinoid field is the study of the potential application of these compounds as antitumoral drugs. Here, we describe the signaling pathway that mediates cannabinoid-induced apoptosis of tumor cells.We identify the stress-regulated protein p8 as an essential mediator of cannabinoid antitumoral action. Activation of this pathway may constitute a potential therapeutic strategy for inhibiting tumor growth.”
The stress-regulated protein p8 mediates cannabinoid-induced apoptosis of tumor cells. Carracedo A, Lorente M, Egia A, Blázquez C, García S, Giroux V, Malicet C, Villuendas R, Gironella M, González-Feria L, Piris MA, Iovanna JL, Guzmán M, Velasco G. Source Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, 28040 Madrid, Spain.

Abstract
One of the most exciting areas of current research in the cannabinoid field is the study of the potential application of these compounds as antitumoral drugs. Here, we describe the signaling pathway that mediates cannabinoid-induced apoptosis of tumor cells. By using a wide array of experimental approaches, we identify the stress-regulated protein p8 (also designated as candidate of metastasis 1) as an essential mediator of cannabinoid antitumoral action and show that p8 upregulation is dependent on de novo-synthesized ceramide. We also observe that p8 mediates its apoptotic effect via upregulation of the endoplasmic reticulum stress-related genes ATF-4, CHOP, and TRB3. Activation of this pathway may constitute a potential therapeutic strategy for inhibiting tumor growth.”
http://www.ncbi.nlm.nih.gov/pubmed/16616335

Down-Regulation of Tissue Inhibitor of Metalloproteinases-1 in Gliomas: A New Marker of Cannabinoid Antitumoral Activity?
“Cannabinoids, the active components of Cannabis sativa L., inhibit tumor growth by inducing apoptosis of tumor cells and inhibiting tumor angiogenesis. We evaluated the effects of cannabinoids on the expression of tissue inhibitors of metalloproteinases (TIMPs), which play critical roles in the acquisition of migrating and invasive capacities by tumor cells. Delta(9)-tetrahydrocannabinol (THC) down-regulated TIMP-1. As TIMP-1 up-regulation is associated with high malignancy and negative prognosis of numerous cancers, TIMP-1 down-regulation may be a hallmark of cannabinoid-induced inhibition of glioma progression.”
Down-regulation of tissue inhibitor of metalloproteinases-1 in gliomas: a new marker of cannabinoid antitumoral activity? Blázquez C, Carracedo A, Salazar M, Lorente M, Egia A, González-Feria L, Haro A, Velasco G, Guzmán M. Source Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, 28040 Madrid, Spain.

Abstract
Cannabinoids, the active components of Cannabis sativa L. and their derivatives, inhibit tumor growth in laboratory animals by inducing apoptosis of tumor cells and inhibiting tumor angiogenesis. It has also been reported that cannabinoids inhibit tumor cell invasiveness, but the molecular targets of this cannabinoid action remain elusive. Here we evaluated the effects of cannabinoids on the expression of tissue inhibitors of metalloproteinases (TIMPs), which play critical roles in the acquisition of migrating and invasive capacities by tumor cells. Local administration of Delta(9)-tetrahydrocannabinol (THC), the major active ingredient of cannabis, down-regulated TIMP-1 expression in mice bearing subcutaneous gliomas, as determined by Western blot and immunofluorescence analyses. This cannabinoid-induced inhibition of TIMP-1 expression in gliomas (i) was mimicked by JWH-133, a selective CB(2) cannabinoid receptor agonist that is devoid of psychoactive side effects, (ii) was abrogated by fumonisin B1, a selective inhibitor of ceramide synthesis de novo, and (iii) was also evident in two patients with recurrent glioblastoma multiforme (grade IV astrocytoma). THC also depressed TIMP-1 expression in cultures of various human glioma cell lines as well as in primary tumor cells obtained from a glioblastoma multiforme patient. This action was prevented by pharmacological blockade of ceramide biosynthesis and by knocking-down the expression of the stress protein p8. As TIMP-1 up-regulation is associated with high malignancy and negative prognosis of numerous cancers, TIMP-1 down-regulation may be a hallmark of cannabinoid-induced inhibition of glioma progression.”
http://www.ncbi.nlm.nih.gov/pubmed/17675107

Inhibition of Cancer Cell Invasion By Cannabinoids Via Increased Expression of Tissue Inhibitor of Matrix Metalloproteinases-1
‎”Cannabinoids, in addition to having palliative benefits in cancer therapy, have been associated with anticarcinogenic effects. Increased expression of TIMP-1 mediates an anti-invasive effect of cannabinoids. Cannabinoids may therefore offer a therapeutic option in the treatment of highly invasive cancers.”
Inhibition of cancer cell invasion by cannabinoids via increased expression of tissue inhibitor of matrix metalloproteinases-1.
Ramer R, Hinz B. Source Institute of Toxicology and Pharmacology, University of Rostock, Schillingallee 70, Rostock D-18057, Germany.

Abstract
BACKGROUND: Cannabinoids, in addition to having palliative benefits in cancer therapy, have been associated with anticarcinogenic effects. Although the antiproliferative activities of cannabinoids have been intensively investigated, little is known about their effects on tumor invasion.

METHODS: Matrigel-coated and uncoated Boyden chambers were used to quantify invasiveness and migration, respectively, of human cervical cancer (HeLa) cells that had been treated with cannabinoids (the stable anandamide analog R(+)-methanandamide [MA] and the phytocannabinoid delta9-tetrahydrocannabinol [THC]) in the presence or absence of antagonists of the CB1 or CB2 cannabinoid receptors or of transient receptor potential vanilloid 1 (TRPV1) or inhibitors of p38 or p42/44 mitogen-activated protein kinase (MAPK) pathways. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to assess the influence of cannabinoids on the expression of matrix metalloproteinases (MMPs) and endogenous tissue inhibitors of MMPs (TIMPs). The role of TIMP-1 in the anti-invasive action of cannabinoids was analyzed by transfecting HeLa, human cervical carcinoma (C33A), or human lung carcinoma cells (A549) cells with siRNA targeting TIMP-1. All statistical tests were two-sided.

RESULTS: Without modifying migration, MA and THC caused a time- and concentration-dependent suppression of HeLa cell invasion through Matrigel that was accompanied by increased expression of TIMP-1. At the lowest concentrations tested, MA (0.1 microM) and THC (0.01 microM) led to a decrease in invasion (normalized to that observed with vehicle-treated cells) of 61.5% (95% CI = 38.7% to 84.3%, P < .001) and 68.1% (95% CI = 31.5% to 104.8%, P = .0039), respectively. The stimulation of TIMP-1 expression and suppression of cell invasion were reversed by pretreatment of cells with antagonists to CB1 or CB2 receptors, with inhibitors of MAPKs, or, in the case of MA, with an antagonist to TRPV1. Knockdown of cannabinoid-induced TIMP-1 expression by siRNA led to a reversal of the cannabinoid-elicited decrease in tumor cell invasiveness in HeLa, A549, and C33A cells.

CONCLUSION: Increased expression of TIMP-1 mediates an anti-invasive effect of cannabinoids. Cannabinoids may therefore offer a therapeutic option in the treatment of highly invasive cancers.”
http://jnci.oxfordjournals.org/content/100/1/59.long

Cannabinoids Inhibit Glioma Cell Invasion by Down-regulating Matrix Metalloproteinase-2 Expression
‎”Local administration of Δ9-tetrahydrocannabinol (THC), the major active ingredient of cannabis, down-regulated MMP-2 expression in gliomas. As MMP-2 up-regulation is associated with high progression and poor prognosis of gliomas and many other tumors, MMP-2 down-regulation constitutes a new hallmark of cannabinoid antitumoral activity.”
Cannabinoids Inhibit Glioma Cell Invasion by Down-regulating Matrix Metalloproteinase-2 Expression Cristina Blázquez1, María Salazar1, Arkaitz Carracedo1, Mar Lorente1, Ainara Egia1, Luis González-Feria2, Amador Haro1, Guillermo Velasco1, and Manuel Guzmán1 
+ Author Affiliations 1Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, Madrid, Spain and 2Department of Neurosurgery, Hospital Universitario de Canarias, La Laguna, Tenerife, Spain  

Abstract
Cannabinoids, the active components of Cannabis sativa L. and their derivatives, inhibit tumor growth in laboratory animals by inducing apoptosis of tumor cells and impairing tumor angiogenesis. It has also been reported that these compounds inhibit tumor cell spreading, but the molecular targets of this cannabinoid action remain elusive. Here, we evaluated the effect of cannabinoids on matrix metalloproteinase (MMP) expression and its effect on tumor cell invasion. Local administration of Δ9-tetrahydrocannabinol (THC), the major active ingredient of cannabis, down-regulated MMP-2 expression in gliomas generated in mice, as determined by Western blot, immunofluorescence, and real-time quantitative PCR analyses. This cannabinoid-induced inhibition of MMP-2 expression in gliomas (a) was MMP-2–selective, as levels of other MMP family members were unaffected; (b) was mimicked by JWH-133, a CB2 cannabinoid receptor–selective agonist that is devoid of psychoactive side effects; (c) was abrogated by fumonisin B1, a selective inhibitor of ceramide biosynthesis; and (d) was also evident in two patients with recurrent glioblastoma multiforme. THC inhibited MMP-2 expression and cell invasion in cultured glioma cells. Manipulation of MMP-2 expression by RNA interference and cDNA overexpression experiments proved that down-regulation of this MMP plays a critical role in THC-mediated inhibition of cell invasion. Cannabinoid-induced inhibition of MMP-2 expression and cell invasion was prevented by blocking ceramide biosynthesis and by knocking-down the expression of the stress protein p8. As MMP-2 up-regulation is associated with high progression and poor prognosis of gliomas and many other tumors, MMP-2 down-regulation constitutes a new hallmark of cannabinoid antitumoral activity.”
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116828/?tool=pubmed

How To Save Yourself From Cancer and Many Other Illnesses With Cannabis

People know of the big C word…Cancer.  When faced with the diagnosis of cancer what do you do, how do you react?  There are conventional treatments for cancer ranging from drugs to radiation to chemotherapy.  Most of the conventional therapies nearly destroy your body along with the cancer. There are some people who have decide to treat cancer in a natural way. When faced with the harsh reality of death as a possibility it can be hard to decide.

Rick Simpson has been providing people with instructions on how to make Hemp Oil medicines for about 8 years.  He has shown how you can treat ailments that are minor to curing cancer.  Below is his presentation RUN FROM THE CURE and other survivor stories that have used the Rick Simpson Oil.  After watching this ask yourself if its time to legalize Cannabis.

RUN FROM THE CURE – Full Version

Grandmother Cancer Survivor Using Rick Simpson Oil

Various Newscasts Pieced Together To Prove Cannabis Cures

You can also read more testimonials Here.  Spread the Truth about the CURE!!
http://phoenixtears.ca/testimonials/

Prostate Cancer and Cannabis:Cause and CURE

Cannabis has been used for thousands of years as a natural remedy for many ailments but only recently has modern science began to recognize the curing power of cannabis.  In 2011 alone there were about 240,890 new cases and about 33,720 deaths from prostate cancer.  Below are studies that show how cannabis can be an effective therapy for prostate cancer.   With more research we can prove that cannabis does have medicinal value and can greatly aid or cure many ailments.  These are published medical studies that I encourage you to research and share your findings with everyone.  Spread the truth!
Information gathered by David Worrell edited by Cherry Girl

Cause:

CXCL12 / CXCR4 / CXCR7 Chemokine Axis And Cancer Progression
‎”CXCL12 / CXCR4 / CXCR7 chemokine axis and cancer progression.” Sun X, Cheng G, Hao M, Zheng J, Zhou X, Zhang J, Taichman RS, Pienta KJ, Wang J. SourceDepartment of Biochemistry and Molecular & Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People’s Republic of China. Erratum in Cancer Metastasis Rev. 2011 Jun;30(2):269-70.

Chemokine Family and Their Cognate Receptors

Abstract
Chemokines, small pro-inflammatory chemoattractant cytokines that bind to specific G-protein-coupled seven-span transmembrane receptors, are major regulators of cell trafficking and adhesion. The chemokine CXCL12 (also called stromal-derived factor-1) is an important α-chemokine that binds primarily to its cognate receptor CXCR4 and thus regulates the trafficking of normal and malignant cells. For many years, it was believed that CXCR4 was the only receptor for CXCL12. Yet, recent work has demonstrated that CXCL12 also binds to another seven-transmembrane span receptor called CXCR7. Our group and others have established critical roles for CXCR4 and CXCR7 on mediating tumor metastasis in several types of cancers, in addition to their contributions as biomarkers of tumor behavior as well as potential therapeutic targets. Here, we review the current concepts regarding the role of CXCL12 / CXCR4 / CXCR7 axis activation, which regulates the pattern of tumor growth and metastatic spread to organs expressing high levels of CXCL12 to develop secondary tumors. We also summarize recent therapeutic approaches to target these receptors and/or their ligands.”
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175097/?tool=pubmed 

Schematic representation showing the role of microenvironment in tumor cell CXCR4 receptor activation in both the primary and metastatic sites.

CURE:

Cannabinoid Receptor CB2 Modulates the CXCL12/CXCR4-Mediated Chemotaxis of T Lymphocytes
‎”Cannabinoid receptor CB2 modulates the CXCL12/CXCR4-mediated chemotaxis of T lymphocytes.
Ghosh S, Preet A, Groopman JE, Ganju RK.
SourceDivision of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA.

Abstract
Cannabinoids have been shown to influence the immune system. However, their immunomodulatory effects have not been extensively studied. In this investigation, we have observed that both primary and Jurkat T cells express a functional cannabinoid receptor 2 (CB(2)). Furthermore, both the synthetic cannabinoids CP55,940 and WIN55,212-2, as well as the CB(2)-selective agonist JWH-015, caused a significant inhibition of the chemokine CXCL12-induced and CXCR4-mediated chemotaxis of Jurkat T cells, as well as their transendothelial migration. Involvement of the CB(2) receptor was further confirmed by partial reversal of the inhibition using the CB(2)-specific antagonist, AM630. Similarly, CP55,940 and JWH-015 inhibited the CXCL12-induced chemotaxis of primary CD4(+) and CD8(+) T lymphocytes. Further investigation of signaling studies to delineate the mechanism of inhibition revealed that cannabinoids enhance CXCL12-induced p44/42 MAP kinase activity. However, enhanced MAP kinase activity was not responsible for the inhibition of chemotaxis. This suggests that cannabinoids differentially regulate CXCR4-mediated migration and MAP kinase activation in T cells. Cannabinoids were also found to downregulate the PMA-enhanced enzyme activity of matrix metalloproteinase-9, which is known to play an important role in transendothelial migration. This study provides novel information regarding cannabinoid modulation of functional effects in T cells.”
http://www.ncbi.nlm.nih.gov/pubmed/16503355

The importance of the CXCL12-CXCR4 chemokine ligand-receptor interaction in prostate cancer metastasis
“These results suggest prostate cancers may be influenced by the CXCL12:CXCR4 pathway during metastasis. This pathway would provide a novel target for therapeutic intervention.”
‎”The importance of the CXCL12-CXCR4 chemokine ligand-receptor interaction in prostate cancer metastasis.  Arya M, Patel HR, McGurk C, Tatoud R, Klocker H, Masters J, Williamson M.  Source Prostate Cancer Research Centre, Institute of Urology,University College London, UK. manit_arya@hotmail.com

Abstract
AIM: Chemokines or chemotactic cytokines are known to be important in the directional migration or chemotaxis of leucocytes in conditions of homeostasis and in inflammatory or immunological responses. However, the role of chemokines is extending beyond their involvement in mediating leucocyte trafficking with an increasing body of evidence suggesting these proteins are intimately involved in many stages of tumour development and progression. Our aim was to study the role of the CXCL12:CXCR4 chemokine ligand:receptor complex in determining the organ-specific metastasis of prostate cancer. MATERIALS and

METHODS: CXCR4 mRNA expression was determined by RT-PCR in 3 metastatic prostate cancer cell lines DU145, LNCaP and PC3, the primary prostate cancer cell line 1542 CPT3X and the normal prostate epithelial cell lines 1542 NPTX and Pre 2.8. This was followed by Taqman quantitative PCR analysis of CXCR4 mRNA in these cell lines. Flow cytometry analysis was then used to measure the expression of the CXCR4 receptor protein on the cell surface. The influence of the receptor on cell migration was studied using Transwell, Migration Assays. Finally, Taqman quantitative PCR was performed on RNA obtained from laser microdissected fresh primary prostate tumour and benign tissue samples from patients.

RESULTS: In DU145, LNCaP and PC3 CXCR4 mRNA expression was approximately 1000, 400 and 21 times respectively that of 1542 NPTX, Pre 2.8 and 1542 CPT3X. In patient primary tumour samples and patient benign tissue specimens CXCR4 mRNA expression was similar to that of the metastatic cell line DU145. Flow cytometry analysis showed that significantly higher levels of the CXCR4 receptor were present on the cell surface of the 3 metastatic cell lines. Migration studies revealed that chemotaxis of the metastatic cell lines PC3 and DU145 was enhanced by CXCL12 ligand and inhibited by antibody to CXCR4. CXCL12 did not influence the migration of the normal prostate epithelial cell line 1542 NPTX.

CONCLUSIONS: We have demonstrated that human prostate cell lines derived from metastases express functional CXCR4 receptor and that CXCL12 ligand enhances their migratory capabilities. Also, laser microdissected primary patient tumours and patient benign tissue specimens express CXCR4 mRNA at high levels (it is suggested that post-transcriptional modification of the CXCR4 receptor plays a major role in regulating protein expression). These results suggest prostate cancers may be influenced by the CXCL12:CXCR4 pathway during metastasis. This pathway would provide a novel target for therapeutic intervention.”
http://www.ncbi.nlm.nih.gov/pubmed/15844659

Toke and Poke:Cannabis and Breast Cancer

October is Breast Cancer Awareness month and its in full swing.  To help celebrate the lives lost as well as saved, we have gathered research that shows cannabis to be a useful treatment option not only for symptoms but for cancer itself.  With more research we can prove that cannabis does have medicinal value and can greatly aid or cure many ailments.  These are published medical studies that I encourage you to research and share your findings with everyone.  Spread the truth!
Information gathered by David Worrell edited by Cherry Girl
Self examination is still a great way for early detection so don’t for get to toke and poke at least once a month.  (Video at bottom or page)

Cause:

Activation of Multiple Cancer-Associated Genes At the ERBB2 Amplicon in Breast Cancer
The ERBB2 (neu/HER2) oncogene, located at chromosome 17q12, is one of the most intensively studied genes in cancer, especially in breast cancer.
Kauraniemi P, Kallioniemi A. SourceLaboratory of Cancer Biology, University of Tampere and Tampere University Hospital, 33014 Tampere, Finland. paivikki.kauraniemi@uta.fi

Abstract
During the past decade the role of the ERBB2 (neu/HER2) oncogene as an important predictor of patient outcome and response to various therapies in breast cancer has been clearly established. This association of ERBB2 aberrations with more aggressive disease and poor clinical outcome, together with the high prevalence of such alterations in breast cancer, has also made ERBB2 an attractive target for therapy. A specific antibody-based therapy, Herceptin, directed against the extracellular domain of the ERBB2 receptor tyrosine kinase, was recently developed and several clinical trials have shown the therapeutic efficacy of this drug against ERBB2-positive breast cancer. However, a relatively large fraction of patients does not benefit from Herceptin treatment, indicating that other factors beyond ERBB2 itself must influence therapy response in ERBB2-positive tumors. It is well known that amplification of the 17q12-q21 region is the most common mechanism for ERBB2 activation in breast cancer and that it leads to simultaneous activation of several other genes. These co-amplified and co-activated genes may have an impact on disease progression and the clinical behavior of ERBB2-positive tumors and thus represent important targets of research. In this paper we discuss the current knowledge on the structure of the ERBB2 amplicon, the genes involved, and their possible contribution to breast cancer pathogenesis.”
http://erc.endocrinology-journals.org/content/13/1/39.long

CURE:

Cannabinoids Reduce ErbB2-Driven Breast Cancer Progression Through Akt Inhibition
“Taken together, these results provide a strong preclinical evidence for the use of cannabinoid-based therapies for the management of ErbB2-positive breast cancer.”
‎”Cannabinoids reduce ErbB2-driven breast cancer progression through Akt inhibition.  Caffarel MM, Andradas C, Mira E, Pérez-Gómez E, Cerutti C, Moreno-Bueno G, Flores JM, García-Real I, Palacios J, Mañes S, Guzmán M, Sánchez C.
SourceDept, Biochemistry and Molecular Biology I, School of Biology, Complutense University, Madrid, Spain.

Abstract
BACKGROUND: ErbB2-positive breast cancer is characterized by highly aggressive phenotypes and reduced responsiveness to standard therapies. Although specific ErbB2-targeted therapies have been designed, only a small percentage of patients respond to these treatments and most of them eventually relapse. The existence of this population of particularly aggressive and non-responding or relapsing patients urges the search for novel therapies. The purpose of this study was to determine whether cannabinoids might constitute a new therapeutic tool for the treatment of ErbB2-positive breast tumors. We analyzed their antitumor potential in a well established and clinically relevant model of ErbB2-driven metastatic breast cancer: the MMTV-neu mouse. We also analyzed the expression of cannabinoid targets in a series of 87 human breast tumors.

RESULTS: Our results show that both Delta9-tetrahydrocannabinol, the most abundant and potent cannabinoid in marijuana, and JWH-133, a non-psychotropic CB2 receptor-selective agonist, reduce tumor growth, tumor number, and the amount/severity of lung metastases in MMTV-neu mice. Histological analyses of the tumors revealed that cannabinoids inhibit cancer cell proliferation, induce cancer cell apoptosis, and impair tumor angiogenesis. Cannabinoid antitumoral action relies, at least partially, on the inhibition of the pro-tumorigenic Akt pathway. We also found that 91% of ErbB2-positive tumors express the non-psychotropic cannabinoid receptor CB2.

CONCLUSIONS: Taken together, these results provide a strong preclinical evidence for the use of cannabinoid-based therapies for the management of ErbB2-positive breast cancer.”

The Expression Level of CB1 and CB2 Receptors Determines Their Efficacy at Inducing Apoptosis in Ast

‎”The high expression level of CB1 and CB2 receptors commonly found in malignant astrocytomas precludes the use of cannabinoids as therapeutics, unless AKT is concomitantly inhibited, or cannabinoids are applied at concentrations that bypass CB1 and CB2 receptors, yet still activate ERK1/2.” So, for astrocytomas, cannabinoids can only be used if they inhibit AKT and activate ERK1/2.
“The expression level of CB1 and CB2 receptors determines their efficacy at inducing apoptosis in astrocytomas. Cudaback E, Marrs W, Moeller T, Stella N.
SourceDepartment of Pharmacology, University of Washington, Seattle, Washington, United States of America.

Abstract
BACKGROUND: Cannabinoids represent unique compounds for treating tumors, including astrocytomas. Whether CB(1) and CB(2) receptors mediate this therapeutic effect is unclear.

PRINCIPAL FINDINGS: We generated astrocytoma subclones that express set levels of CB(1) and CB(2), and found that cannabinoids induce apoptosis only in cells expressing low levels of receptors that couple to ERK1/2. In contrast, cannabinoids do not induce apoptosis in cells expressing high levels of receptors because these now also couple to the prosurvival signal AKT. Remarkably, cannabinoids applied at high concentration induce apoptosis in all subclones independently of CB(1), CB(2) and AKT, but still through a mechanism involving ERK1/2.

SIGNIFICANCE: The high expression level of CB(1) and CB(2) receptors commonly found in malignant astrocytomas precludes the use of cannabinoids as therapeutics, unless AKT is concomitantly inhibited, or cannabinoids are applied at concentrations that bypass CB(1) and CB(2) receptors, yet still activate ERK1/2.”
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806825/?tool=pubmed

Cannabidiol Induces Programmed Cell Death In Breast Cancer Cells By Coordinating the Cross-Talk Between Apoptosis and Autophagy
‎”Cannabidiol induces programmed cell death in breast cancer cells by coordinating the cross-talk between apoptosis and autophagy. Cannabidiol (CBD), a major nonpsychoactive constituent of cannabis, is considered an antineoplastic agent on the basis of its in vitro and in vivo activity against tumor cells.”
“Cannabidiol induces programmed cell death in breast cancer cells by coordinating the cross-talk between apoptosis and autophagy.
Shrivastava A, Kuzontkoski PM, Groopman JE, Prasad A. Source Division of Experimental Medicine, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA. abailugu@bidmc.harvard.edu

Abstract
Cannabidiol (CBD), a major nonpsychoactive constituent of cannabis, is considered an antineoplastic agent on the basis of its in vitro and in vivo activity against tumor cells. However, the exact molecular mechanism through which CBD mediates this activity is yet to be elucidated. Here, we have shown CBD-induced cell death of breast cancer cells, independent of cannabinoid and vallinoid receptor activation. Electron microscopy revealed morphologies consistent with the coexistence of autophagy and apoptosis. Western blot analysis confirmed these findings. We showed that CBD induces endoplasmic reticulum stress and, subsequently, inhibits AKT and mTOR signaling as shown by decreased levels of phosphorylated mTOR and 4EBP1, and cyclin D1. Analyzing further the cross-talk between the autophagic and apoptotic signaling pathways, we found that beclin1 plays a central role in the induction of CBD-mediated apoptosis in MDA-MB-231 breast cancer cells. Although CBD enhances the interaction between beclin1 and Vps34, it inhibits the association between beclin1 and Bcl-2. In addition, we showed that CBD reduces mitochondrial membrane potential, triggers the translocation of BID to the mitochondria, the release of cytochrome c to the cytosol, and, ultimately, the activation of the intrinsic apoptotic pathway in breast cancer cells. CBD increased the generation of reactive oxygen species (ROS), and ROS inhibition blocked the induction of apoptosis and autophagy. Our study revealed an intricate interplay between apoptosis and autophagy in CBD-treated breast cancer cells and highlighted the value of continued investigation into the potential use of CBD as an antineoplastic agent.”
http://www.ncbi.nlm.nih.gov/pubmed/21566064

Medical Marijuana Stops Spread of Breast Cancer – NBC NEWS